SNP microarray-based 24 chromosome aneuploidy screening is significantly more consistent than FISH
نویسندگان
چکیده
منابع مشابه
SNP microarray-based 24 chromosome aneuploidy screening is significantly more consistent than FISH
Many studies estimate that chromosomal mosaicism within the cleavage-stage human embryo is high. However, comparison of two unique methods of aneuploidy screening of blastomeres within the same embryo has not been conducted and may indicate whether mosaicism has been overestimated due to technical inconsistency rather than the biological phenomena. The present study investigates the prevalence ...
متن کاملSNP microarray-based 24 chromosome aneuploidy screening demonstrates that cleavage-stage FISH poorly predicts aneuploidy in embryos that develop to morphologically normal blastocysts
Although selection of chromosomally normal embryos has the potential to improve outcomes for patients undergoing IVF, the clinical impact of aneuploidy screening by fluorescence in situ hybridization (FISH) has been controversial. There are many putative explanations including sampling error due to mosaicism, negative impact of biopsy, a lack of comprehensive chromosome screening, the possibili...
متن کاملDevelopment and validation of a next-generation sequencing-based protocol for 24-chromosome aneuploidy screening of embryos.
OBJECTIVE To validate a next-generation sequencing (NGS)-based method for 24-chromosome aneuploidy screening and to investigate its applicability to preimplantation genetic screening (PGS). DESIGN Retrospective blinded study. SETTING Reference laboratory. PATIENT(S) Karyotypically defined chromosomally abnormal single cells and whole-genome amplification (WGA) products, previously analyze...
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The present study evaluated the role of SNP microarray in 101 cases of clinically suspected FISH negative (noninformative/normal) 22q11.2 microdeletion syndrome. SNP microarray was carried out using 300 K HumanCytoSNP-12 BeadChip array or CytoScan 750 K array. SNP microarray identified 8 cases of 22q11.2 microdeletions and/or microduplications in addition to cases of chromosomal abnormalities a...
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The publication in the mid-1990s of a new molecular tool, the DNA microarray, has led to a revolution in the way scientists approach the investigation of gene expression and regulation. This technology has made its impact upon many basic scientific disciplines including cancer biology, developmental biology, toxicology, investigation of growth-factor and hormonal signaling, and the applied area...
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ژورنال
عنوان ژورنال: Molecular Human Reproduction
سال: 2010
ISSN: 1360-9947,1460-2407
DOI: 10.1093/molehr/gaq039